mrc-5 human normal embryonal lung fibroblast cell line  (LGC Promochem)

Journal: PLoS ONE

Article Title: Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

doi: 10.1371/journal.pone.0155725

Figure Lengend Snippet: (A) Quantification of mean number of SA-β-Gal positive cells in A375, MRC5 and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.

Article Snippet: Human malignant melanoma A375 cells (A375) and human normal embryonic lung fibroblast cell line MRC5 [ ] were purchased from the American Type Culture Collection.

Techniques: Irradiation, Labeling, Expressing, Western Blot, Activation Assay, Positive Control, Control, Fluorescence, Staining